Moringa Leaf Extract Improves Spermatozoa Motility and Viability Through an Antioxidant Mechanism in Older Wistar Rats

Luh Putu Widiastini; I Gusti Agung Manik Karuniadi

doi:10.14334/jitv.v30i4.3405

Moringa Leaf Extract Improves Spermatozoa Motility and Viability Through an Antioxidant Mechanism in Older Wistar Rats

Luh Putu Widiastini, I Gusti Agung Manik Karuniadi

DOI

https://doi.org/10.14334/jitv.v30i4.3405

Abstract

The reproductive aging is characterized by a progressive decline in physiological integrity that triggers in damaging to the testicle, epididymis and other reproductive organs. An oxidative stress is associated with aging and infertility in men which causes a decrease in semen volume, abnormal morphology, decreased sperm viability and motility. One of the treatments for oxidative stress is the use of antioxidant compounds such as Moringa plants. The purpose of this study was to determine the antioxidant mechanism of moringa leaf ethanol extract in sperm motility and viability in older Wistar rats. This study is a randomized post-test only control group design. The total sample was 36 older Wistar rats (age 18–19 months), which were divided randomly into 2 groups, including the treatment group (18 rats) was given ethanol extract of Moringa oleifera (EEMO) 50 mg/kg BW+CMC 0.5%, 0.5 ml /day, and the control group (18 rats) was given CMC 0.5%, 0,5 ml/day, for 30 days. Rats were terminated by cervical dislocation method. Motility and viability of spermatozoa were examined. Data analysis using an independent sample t-test and Mann Whitney with a significance value of P= 0.5. The results showed that the antioxidant content of EEMO 50 mg/kg BW+CMC 0.5%, for 30 days in old Wistar rats increased progressive motility, decreased non-progressive motility and immotility, and increased spermatozoa viability, significantly in the treatment group compared to the control group with a P<0.05. In conclusion, Moringa leaf extract could improves spermatozoa motility and viability through an antioxidant mechanism in old wistar rats.

Keywords

AntioxidaAntioxidant; Moringa Leaf; Motility; Old Rat; Viabilitynt, Moringa Leaf, Motility, Viability, old rat

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