Influence of Different Rearing Systems and Sex on the Hematological Profile of IPB D3 Chickens

Wawan Kuswandi; I Khaerunnisa; C Budiman; Cece Sumantri

doi:10.14334/jitv.v30i4.3519

Influence of Different Rearing Systems and Sex on the Hematological Profile of IPB D3 Chickens

Wawan Kuswandi, I Khaerunnisa, C Budiman, Cece Sumantri

DOI

https://doi.org/10.14334/jitv.v30i4.3519

Abstract

Rearing systems significantly influence the welfare, productivity, and physiological status of poultry, including hematological responses that reflect immune and health conditions. This study analysed the interaction between rearing system and sex on the hematological profile of IPB-D3 chicken. A total of 60 twelve-week-old chickens were reared for 12 weeks under two systems: intensive and free-range, with 30 birds per system (15 males and 15 females). At 24 weeks of age, blood samples were collected from the brachial vein into EDTA tubes. Hematological analysis was performed using standard methods, including erythrocyte and leukocyte counts using a hemocytometer, hemoglobin determination, hematocrit measurement by the microhematocrit method, and differential leukocyte counts using blood smears stained with Giemsa stain. Results showed that erythrocyte count, hemoglobin concentration, and erythrocyte indices did not differ significantly between rearing systems (P>0.05). Hematocrit was not significantly affected by rearing system alone, but a significant interaction between system and sex was observed (P= 0.038), with free-range males exhibiting higher hematocrit than intensively reared males. Total leukocyte count was significantly influenced by rearing system (P= 0.010), with intensively reared chickens showing higher leukocyte levels (19.52×10³/µL) than free-range chickens (14.12×10³/µL), without interaction with sex. Differential leukocyte percentages and the heterophil-to-lymphocyte ratio were not significantly different (P>0.05). In conclusion, although certain hematological parameters were influenced by rearing system and its interaction with sex, IPB-D3 chicken demonstrated good physiological adaptability under both intensive and free-range conditions.

Keywords

Free-Range System; Hematological Profile; IPB-D3 Chickens; Rearing System; Sex Interaction

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